Parallel Functional Protein Analysis

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Applications

• Target protein identification, using antibodies and sera ^1,2
• Functional assays, e.g. phosphorylation, ribosylation, methylation ^3,4
• Identification of DNA/RNA binding proteins ¹
  
• Identification of protein−protein interactions^9,10

Product Range

• Custom-made Protein Arrays can be generated from subsets of these libraries as well as from any expression library provided by the customer.
• Target protein identification, using antibodies and sera is offered as a standard service.
• Other applications, such as functional assays, e.g. phosphorylation, ribosylation, methylation, and identification of DNA/RNA-binding proteins are provided as special services upon request.
• DNA sequences encoding for identified proteins are provided upon request.
  **Note: Human Testis is not sequenced, clones have to be ordered for sequencing by the customer

Product Details

References

For detailed information about the generation, characterization and application of the Protein Expression Libraries please click here.
The generation of Protein Arrays is based on a technology licensed from Prot@gen AG.

1.	G. Grelle, S. Kostka, A. Otto, B. Kersten, K. Genser, E. Müller, S. Wälter, A. Böddrich, U. Stelzl, C. Hänig,  R. Volkmer-Engert, C. Landgraf, S. Alberti, J. Höhfeld, M. Strödicke, and E. Wanker: Identification of VCP/p97, CHIP and amphiphysin II interaction partners using membrane-based human proteome arrays. MCP (2005) 10.1074/mcp.M500198−MCP200
2.	K. de Graaf, P. Hekerman, O. Spelten, A. Herrmann, L. C. Packman, K. Bussow. G. Muller-Newen, W. Becker (2004) : Characterization of cyclin L2, a novel cyclin with an arginine/serine-rich domain: phosphorylation by DYRK1A and colocalization with splicing factors. J Biol Chem. 279(6):4612−24
3.	K. Büssow, C. Quedenau, V. Sievert, J. Tischler, C. Scheich, H. Seitz, B. Hieke, F.H. Niesen, F. Gotz, U. Harttig, H. Lehrach: A catalog of human cDNA expression clones and its application to structural genomics. Genome Biol. 2004 Aug; 5(9):R71
4.	U. Radelof, C.Hüls, B. Korn, J. Maurer: Proteinarrays und rekombinante Proteine für die Proteinanalyse. Laborwelt 2004. 5: p.35
5.	C. Maercker: Protein-Chips in der Genomforschung. Laborwelt 2004. 4: p.12−15
6.	J. Lee and M.T. Bedford: PABP1 identified as an arginine methyltransferase substrate using high-density protein arrays. EMBO Reports 2002. 3(3): p.268−73
7.	U. Mahlknecht, O.G. Ottmann, and D. Hoelzer: Far-Western based protein-protein interaction screening of high-density protein filter arrays. Journal of Biotechnology 2001. 88(2): p. 89−94
8.	K. Büssow, E. Nordhoff, C. Lübbert, H. Lehrach, and G. Walter: A human cDNA library for high-throughput protein expression screening. Genomics 2000.65(1): 1−8
9.	L. J. Holt, K. Büssow, G. Walter, and I. M. Tomlinson: By-passing selection: direct screening for antibody−antigen interactions using protein arrays. Nucleic Acids Research 2000. 28(15): p. E72
10.	K. Büssow, D. Cahill, W. Nietfeld, D. Bancroft, E. Scherzinger, H. Lehrach, and G. Walter: A method for global protein expression and antibody screening on high-density filters of an arrayed cDNA library. Nucleic Acids Research 1998. 26(21): p. 5007−5008