imaGenes is a distributor of clone resources produced by the I.M.A.G.E.
Consortium,
which shares high-quality, arrayed cDNA libraries and places sequence,
map, and expression data on the clones in these arrays into the public
domain. All I.M.A.G.E clones are available free of any royalties, and may
be used by anyone agreeing with the I.M.A.G.E. guidelines.
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| MGC, XGC, ZGC predicted full-length |
human, mouse, rat, zebrafish, X.laevis, X.tropicalis |
IRAK |
amp |
+ |
+ |
predicted |
| IRAL |
cam |
+ |
+ |
predicted |
| IRAM |
kan |
+ |
+ |
predicted |
| MGC verified full-length, human |
human |
IRAT |
amp |
+ |
+ |
verified |
| IRAU |
cam |
+ |
+ |
verified |
| MGC verified full-length, mouse |
mouse |
IRAV |
amp |
+ |
+ |
verified |
| IRAW |
cam |
+ |
+ |
verified |
| MGC verified full-length, rat |
rat |
IRBP |
amp |
+ |
+ |
verified |
| IRBQ |
cam |
+ |
+ |
verified |
| XGC verified full-length, Xenopus |
X. laevis |
IRBG |
cam |
+ |
+ |
verified |
| IRBH |
amp |
+ |
+ |
verified |
| X. tropicalis |
IRBN |
amp |
+ |
+ |
verified |
| ZGC verified full-length, zebrafish |
zebrafish |
IRBO |
amp |
+ |
+ |
verified |
| Human genes (lymphochip) |
human |
IRAY |
amp |
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| IRAZ |
cam |
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| IRBA |
amp |
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| IRBB |
cam |
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| Human GPCR genes |
human |
IRBF |
amp |
underway |
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| IRBI |
amp |
underway |
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| Human genes (glioma-related) |
human |
IRAX |
amp |
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| Mouse genes (lymphochip) |
mouse |
IRAP |
amp |
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| Mouse pancreas genes |
mouse |
IRBD |
amp |
underway |
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| IRBE |
kan |
underway |
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| University of IOWA Rat Unigene Set |
rat |
IRAD |
amp |
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Resistance: amp - ampicillin, cam - chloramphenicol, kan - kanamycin
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These human, mouse, rat, zebrafish, Xenopus laevis and X. tropicalis clones have been computationally predicted to be full-length based on homology with known
genes, and several other methods of prediction for novel genes. In 384-well format, the IRAK rearray contains
ampicillin-resistant clones and the IRAL rearray contains
chloramphenicol-resistant clones, and IRAM contains
kanamycin-resistant clones. Each plate contains clones from multiple libraries, but from one species
and one vector only. These clones are being sequence-verified and fully sequenced by the MGC
Consortium. To access the full-length
sequences, search Genbank (not dbEST) using the following format: "MGC:MGC_id" to return individual
sequences or MGC [kywd] for all sequences.
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This is a subset of the IRAK/IRAL MGC predicted full-length set; these clones have been sequence-verified to match the EST already in dbEST, and
have been fully sequenced to high accuracy by the MGC Consortium (full-length sequences are submitted to the primate division of
Genbank, NOT dbEST). This rearray is in 96-well format, with each plate containing clones from one species and
vector only. Rearray names are IRAT
(human clones, ampicillin-resistant vectors) and IRAU (human clones, chloramphenicol-resistant vectors. Periodic updates will
be made as more full-length sequences are submitted. To access the full-length sequences, search Genbank (not
dbEST) using the following format: "MGC:MGC_id" to return individual sequences or MGC [kywd] for all
sequences.
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This is a subset of the IRAK/IRAL MGC predicted full-length set; these clones have been sequence-verified to
match the EST already in dbEST, and have been fully sequenced to high accuracy by the MGC Consortium
(full-length sequences are
submitted to the primate division of Genbank, NOT dbEST). This rearray is in 96-well format, with each plate
containing clones from one species and vector only. Rearray names are IRAV (mouse clones, ampicillin-resistant
vectors) and IRAW (mouse clones,
chloramphenicol-resistant vectors). Periodic updates will be made as more full-length sequences are submitted.
To access the full-length sequences, search Genbank (not dbEST) using the following format:
"MGC:MGC_id" to return individual sequences or MGC [kywd] for all sequences.
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This is a subset of the IRAK/IRAL MGC predicted full-length set; these clones have been sequence-verified to
match the EST already in dbEST, and have been fully sequenced to high accuracy by the MGC Consortium
(full-length sequences are
submitted to the primate division of Genbank, NOT dbEST). This rearray is in 96-well format, with each plate
containing clones from one species and vector only. Rearray names are IRBP (rat clones, ampicillin-resistant
vectors) and IRBQ (rat clones, chloramphenicol resistant vectors). Periodic updates will be made as more full-length sequences are submitted.
To access the full-length sequences, search Genbank (not dbEST) using the following format:
"MGC:MGC_id" to return individual sequences or MGC [kywd] for all sequences.
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This is a subset of the IRAK/IRAL XGC predicted full-length set for X. laevis and X. tropicalis; these clones have been sequence-verified to
match the EST already in dbEST, and have been fully sequenced to high accuracy by XGC (full-length sequences are
submitted to the primate division of Genbank, NOT dbEST). This rearray is in 96-well format, with each plate
containing clones from one species and vector only. Rearray names are IRBG (X. laevis, chloramphenicol-resistant
vectors), IRBH (X. laevis,
ampicillin resistant-vectors) and IRBN
(X. tropicalis, ampicillin-resistant vectors). Periodic updates will be made as more full-length sequences are submitted.
To access the full-length sequences, search Genbank (not dbEST) using the following format:
"MGC:MGC_id" to return individual sequences or MGC [kywd] for all sequences.
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This is a subset of the IRAK/IRAL ZGC predicted full-length set for zebrafish; these clones have been sequence-verified to
match the EST already in dbEST, and have been fully sequenced to high accuracy by ZGC (full-length sequences are
submitted to the primate division of Genbank, NOT dbEST). This rearray is in 96-well format, with each plate
containing clones from one species and vector only. Rearray name is IRBO (zebrafish clones, ampicillin-resistant
vectors). Periodic updates will be made as more full-length sequences are submitted.
To access the full-length sequences, search Genbank (not dbEST) using the following format:
"MGC:MGC_id" to return individual sequences or MGC [kywd] for all sequences.
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This set of 2,500 known human genes (1,415 unique genes, 1,100 of them represented by two independent clones) has been
rearrayed in 96-well format. Each plate contains one vector only. IRAY is a non-redundant rearray of
ampicillin-resistant clones, IRAZ is a
non-redundant rearray of chloramphenicol-resistant clones, IRBA is a non-redundant rearray of ampicillin-resistant clones that completely overlaps the genes
represented in IRAY, and IRBB is a
non-redundant rearray of chloramphenicol-resistant clones that completely overlaps the genes represented in IRAZ.
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The full open reading frames of individual GPCR genes have been
PCR-amplified and cloned in 96-well format. Originally the rearray
names were IRBF
( ampicillin-resistant, from the Guthrie cDNA Resource Center) and IRBI ( ampicillin-resistant, from
the GPCR Consortium, but more recently clones from Guthrie and GPCR Consortium have been combined into single plates under the
IRBI collection. The origin of each clone can still be tracked with the Guthrie clones originating from library NIH_MGC_146
and the GPCR Consortium clones originating from library NIH_MGC_145. For specific information, please refer to the appropriate IMAGE ftp-site.
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A set of 5,500 mostly unique human
ampicillin-resistant clones representing glioma-related genes (rearray name IRAX).
Each plate contains one vector only except for plate 1 which contains a
small number of clones from three different vectors. For specific
information, please refer to the appropriate IMAGE ftp-site.
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A set of unique ampicillin-resistant clones, representing 13,000 mouse genes (rearray name IRAP. Sequence-verification is
planned. Each plate contains one vector only. For specific information, please refer to the appropriate IMAGE ftp-site.
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This is a set of 7,000 mouse genes. Rearray IRBD contains ampicillin-resistant
clones, rearray IRBE contains
kanamycin-resistant clones. For specific information, please refer to the appropriate IMAGE ftp-site.
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The IRAD rearray contains
ampicillin-resistant clones in 96-well format. It was prepared in the Soares laboratory at the University of Iowa based
on Unigene clustering.
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Tel: +49 30 9489 2444
Tel: +49 30 9489 2462
Fax: +49 30 9489 2449
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