Why Synthetic Genes?

Synthetic Genes are open reading frame sequences from publicly annotated genes generated synthetically. A Synthetic Gene is the product of choice, if no natural clone is available or if available clones do not fulfil your specific requirements, such as: 100% sequence identity to a database sequence, DNA sequence optimization for maximization of protein yield in your preferred expression system, e.g. E.coli or yeast.

Search and Order

Synthetic Genes can be readily identified and ordered via imaGenes' intuitive-to-use online search tool GenomeCube®. Alternatively, please contact Sales. You may send us various Accession Ids corresponding to nucleotide sequences (e.g. GenBank, Ensembl) as well as protein Ids (e.g. UniProt). Usually they will be delivered within 4-6 weeks depending on the length of the synthetic construct.

Application

Synthetic Genes are of primary interest for

• Genes for which no clones are available or hard to obtain otherwise (e.g. Full ORF clones, splice variants)
• Increased expression yields due to the sequence optimization
• Design of the sequence due to your specific requirements (e.g. introduction of point mutations, insertions or deletions, epitope and domain shuffling, introduction of fusion tags and particular restriction sites)

Product Range

• Protein expression system specific sequence optimization in order to maximize protein yield
• Gene synthesis
• Generation of gene variants or randomized libraries for structural or functional analyses
• Cloning of synthetic gene into the vector of your choice
• Comprehensive quality control including full-length sequencing
• Protein expression, purification and characterization as a separate service. More

As a technology/source independent provider of Synthetic Genes, imaGenes is able to support your survey for the most economic gene synthesis approach that fits your scientific requirements best. In order to do so, we offer you an in-depth analysis of your requirements in the course of a counseling interview. As a result we provide you with a detailed offer reflecting your needs and the best fitting and most economic approach yet available on the international market. Contact

Disclaimer

imaGenes reserves the right to withdraw from a gene synthesis order if the encoding sequence comprises hazardous, complex or genetically instable sequence elements.