Advantages of ChIP Analysis by Next Generation Sequencing technology

• True whole genome wide discovery of protein-binding sites on DNA is possible, also without comprehensive previous knowledge of genomic sequences 
• Excellent dynamic range and intuitive quantification opportunities, simply by counting the frequency of reads that map to (or establish) the same region 
• Deep sequencing coverage enables statistically meaningful analysis of results (over 5 millions different fragments can be analyzed in a single flow cell channel)
• Fine resolution of binding sites to stretches
• Low sample input amount required  (50 ng of fragmented, ChIP-enriched DNA)

Our Service Offering:

• Illumina DNA Cluster Library generation from ChIP enriched DNA with QC, subsequent sequencing on Genome Analyzer
• Methylation analysis via immunoprecipitation of Methylcytosin-binding proteins, starting with MeDIP enriched DNA 
• Bioinformatic Analysis:  Clustering, mapping to reference genome, quantitation

1. Hudson and Snyder: High-throughput methods of regulatory element discovery; Biotechniques 2006, Vol.41/6, 673-681
2. Fields: Site-seeing by sequencing; Science 2007, 316(5830), 1441-1442