General Information

Applications

• Serum Sceening: Arrayed proteins are hybridised with (a minimum of 100 µl of customer-provided) human patient sera, to find auto-antibodies against targets related to disease, in e.g. autoimmune diseases.


• Antibody Epitope Mapping: Arrayed proteins are incubated with monoclonal or purified antibodies (provided by customer). Positive signals can be used to determine which epitope is recognized by the antibody. This approach is valuable for all clinicians and researchers who would like to work with antibodies that are not fully characterized, especially for developing new antibodies for diagnostics or treatments.

Screening

Patient serum (or plasma) or monoclonal or purified antibody, respectively, is incubated with the desired imaGenes Protein Array. Recognized proteins may be visualized with an ECF-labeled secondary antibody. Customer may either perform experiments on his or her own or employ imaGenes for scanning of proteins and analysis of results. imaGenes recommends to perform at least one replicate of the experiment. Identified proteins and corresponding clones can be provided as annotated clone lists, including EST sequence information, gene symbol, EnsEMBL-gene ID, UniGene cluster ID, protein structure information (if available), etc. Protein Array analysis images and all quality-control results are electronically documented.

References

For detailed information about the generation, characterization and application of the Protein Expression Libraries please click here.
The generation of Protein Arrays is based on a technology licensed from Prot@gen AG.

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6.	J. Lee and M.T. Bedford: PABP1 identified as an arginine methyltransferase substrate using high-density protein arrays. EMBO Reports 2002. 3(3): p.268−73
7.	U. Mahlknecht, O.G. Ottmann, and D. Hoelzer: Far-Western based protein-protein interaction screening of high-density protein filter arrays. Journal of Biotechnology 2001. 88(2): p. 89−94
8.	K. Büssow, E. Nordhoff, C. Lübbert, H. Lehrach, and G. Walter: A human cDNA library for high-throughput protein expression screening. Genomics 2000.65(1): 1−8
9.	L. J. Holt, K. Büssow, G. Walter, and I. M. Tomlinson: By-passing selection: direct screening for antibody−antigen interactions using protein arrays. Nucleic Acids Research 2000. 28(15): p. E72
10.	K. Büssow, D. Cahill, W. Nietfeld, D. Bancroft, E. Scherzinger, H. Lehrach, and G. Walter: A method for global protein expression and antibody screening on high-density filters of an arrayed cDNA library. Nucleic Acids Research 1998. 26(21): p. 5007−5008